Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Chicken Thiobarbituric Acid Reactive Substance (also known as TBARS) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Chicken TBARS antibody. TBARS present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Chicken TBARS Antibody is added and binds to TBARS in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TBARS antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Chicken TBARS. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm